TECHNICAL BULLETINS & Procedures

---

Methods  The 3D cell invasion assay was performed based on the method described by Berens et al. (J. Vis. Exp. (105), e53409, doi:10.3791/53409 (2015)). The BT549 breast cancer cells (ATCC HTB-122) that are highly invasive were used in the assay. The 3D matrix used in the invasion assay was prepared by mixing either OviGenex collagen from or Type I collagen (rat tail) from Vendor with Corning Matrigel GFR Membrane Matrix (Corning, Cat: 356230). While the Vendor collagen was used at 2 mg/mL final concentration as recommended by the manufacturer, OviGenex collagen was used at various concentrations. The cell spheres were imbedded in the 3D matrix and the invasiveness of the cells was analyzed by measuring the expansion of cell front lines. The diagram above  illustrates the experimental procedure.